![]() ![]() High-throughput sequencing including RNA-seq and microarray is a novel technique, which plays an important role in the exploration for genome‐level differences and is providing valuable insights into the landscape of the identification of key genes and functional pathways associated with osteogenic differentiation in BMSCs ( Fan et al., 2020). However, relying solely on the experimental identification of genes that regulate osteogenic differentiation of BMSCs is generally costly and time-consuming, which leads to an urgent demand to develop a prediction method to infer osteogenic differentiation–related genes in a short time. As a key contributor to the bone formation, BMSCs are regulated by genetic factors ( Zhang et al., 2018). These diseases, closely related to the osteogenic differentiation of bone marrow–derived mesenchymal stem cells (BMSCs), have been confirmed by numerous studies ( Marongiu et al., 2020 Xiao et al., 2020 Xiong et al., 2020 Jiang et al., 2021).īMSCs possess self-renewal capabilities and the potential to differentiate into a variety of cell types, including osteoblasts, chondrocytes, and adipocytes ( Bianco et al., 2001 Liao et al., 2017). The rate of bone defects, fracture nonunion, and osteoporosis incidence continues to rise, and available therapeutic options remain limited. Moreover, our method has ability in discriminating genes with osteogenic differentiation properties and can facilitate the process of discovery of new osteogenic differentiation–related genes.Ĭonclusion: These findings collectively demonstrate that AOX1 is an osteogenic differentiation-relevant gene and provide a novel method established with a good performance for osteogenic differentiation-relevant genes prediction. The experiments revealed that AOX1 level was higher and increased gradually in differentiated BMSCs compared with undifferentiated BMSCs, and AOX1 overexpression significantly increased the expression of osteo-specific genes, thereby clearly indicating that AOX1 plays an important role in osteogenic differentiation. GO enrichment analysis and GSEA show that AOX1 is significantly associated with osteoblast-related pathways. Association analysis and PPI network analysis among these differentially expressed genes show that AOX1 is a potential regulator of osteogenic differentiation. Result: We identified 25 upregulated genes and 17 downregulated genes. qRT-PCR and Western blotting were employed to investigate the expression of genes on osteogenic differentiation, and plasmid transfection was used to overexpress the gene AOX1 in hBMSCs. GO enrichment analysis and GSEA are performed to identify significantly enriched pathways associated with AOX1. Association analysis, co-expression analysis, and PPI analysis are then carried out to identify potential osteogenesis-related regulators. Method: We performed differential expression analysis between hBMSCs and osteogenically induced samples. Thus, there remains an urgent demand to develop a prediction method to infer osteogenic differentiation–related genes in BMSCs. 5Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, Chinaīackground: The available therapeutic options of bone defects, fracture nonunion, and osteoporosis remain limited, which are closely related to the osteogenic differentiation of bone marrow–derived mesenchymal stem cells (BMSCs). ![]() 4Orthopedics Research Institute of Zhejiang University, Hangzhou, China.3Department of Orthopedics Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.2Key Laboratory of Image Information Processing and Intelligent Control, School of Artificial Intelligence and Automation, Huazhong University of Science and Technology, Wuhan, China.1Affiliated Hangzhou Xixi Hospital, Zhejiang University School of Medicine, Hangzhou, China.Lingtong Sun 1 † Jianfei Ma 2 † Juan Chen 1 Zhijun Pan 3,4,5* Lijun Li 3,4,5* ![]()
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